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KMID : 0381120210430060623
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Genes and Genomics
2021 Volume.43 No. 6 p.623 ~ p.631
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Paeoniflorin reduces the inflammatory response of THP-1 cells by up?regulating microRNA-124
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Huang Danyun
Li Zhijun
Chen Yue
Fan Yan
Yu Tao
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Abstract
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Background: The activation of macrophages and the release of inflammatory cytokines are the main reasons for the progress of systemic lupus erythematosus (SLE). MicroRNA (miRNA)-124 is involved in the regulation of macrophages and is a key regulator of inflammation and immunity.
Objective: To explore whether paeoniflorin (PF) regulates the biological functions of macrophages depends on miR-124.
Methods: RT-PCR, WB, ELISA, CCK-8 and flow cytometry were used to evaluate that PF regulated the biological functions of THP-1 cells through miR-124.
Results: PF significantly inhibited the proliferation while promotes the apoptosis of THP-1 cells, and inhibited the release of IL-6, TNF-α and IL-1βin THP-1 cells. RT-PCR results shown that PF up-regulated the expression of miR-124 in THP-1 cells. Functional recovery experiments showed that compared with the LPS?+?mimic-NC group, LPS?+?miR-124 mimic significantly inhibited the proliferation and the release of IL-6, TNF-α and IL-1β, but promoted the apoptosis of THP-1 cells. In addition, compared with the LPS?+?PF?+?inhibitor-NC group, LPS?+?PF?+?miR-124 inhibitor significantly promoted the proliferation and the release of IL-6, TNF-α and IL-1β, but inhibited the apoptosis of THP-1 cells.
Conclusions: By down-regulating miR-124, PF inhibits the proliferation and inflammation of THP-1 cells, and promotes the apoptosis of THP-1 cells.
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KEYWORD
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Paeoniflorin, THP-1 cells, miRNA-124, Proliferation, Apoptosis
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